Draft Genome Sequence of Rhodococcus opacus Strain M213 Shows a Diverse Catabolic Potential
January/February 2013
Authors:
Ashish Pathak, Stefan J. Green, Andrew Ogram, Ashvini Chauhana
Info:
Rhodococcus strains have diverse and unusual metabolic pathways that are being considered for industrial roles in bioproduction of chemicals and fuels. The authors present the whole genome sequence of one such strain. Nextera chemistry was used with size selection on the Pippin Prep to generate the sequencing libraries.
Citation:
genomeA (1)1:e00144-12 January/February 2013
A genome-wide survey of genetic variation in gorillas using reduced representation sequencing
January 2013
Authors:
Aylwyn Scally, Bryndis Yngvadottir, Yali Xue, Qasim Ayub, Richard Durbin, Chris Tyler-Smith
Info:
The study used reduced representation libraries to study diversity among the two currently recognized species of gorilla. The libraries were generated by AluI digestion followed by size selection on the Pippin Prep for AluI fragments between 150-250 bp.
Citation:
pre-print
Use of microarray hybrid capture and next-generation sequencing to identify the anatomy of a transgene
January 2013
Authors:
Amanda J. DuBose, Stephen T. Lichtenstein, Narisu Narisu, Lori L. Bonnycastle, Amy J. Swift, Peter S. Chines and Francis S. Collins
Info:
The authors designed a general method to study the arrangement of foreign DNA and host DNA rearrangements in trangenic animals. The protocol involves standard Illumina PE library generation, using the Pippin Prep for size selection, followed by microarray hybridization capture to isolate fragments containing the transgene.
Citation:
Nucleic Acids Research, 2013, 1-5
Discrete genetic modules are responsible for complex burrow evolution in Peromyscus mice
January 2013
Authors:
Jesse N. Weber, Brant K. Peterson & Hopi E. Hoekstra
Info:
The work seeks to identify genetic influences on a complex behavior in mice, burrow formation. The authors used a modified RADseq procedure to genotype two species of wild mice with distinct burrowing behavior. Pippin Prep was used for size selection in their RADseq protocol.
Citation:
Letter, Nature 493:402-405
A Hybrid Genetic Linkage Map of Two Ecologically and Morphologically Divergent Midas Cichlid Fishes(Amphilophus spp.) Obtained by Massively Parallel DNA Sequencing (ddRADSeq)
January 2013
Authors:
Hans Recknagel, Kathryn R. Elmer, and Axel Meyer
Info:
RADseq was used to develop linkage maps for two highly divergent Cichlid fish. Pippin Prep was used to size select the libraries.
Citation:
G3 (Bethesda) 3(1):65-74 January 2013