Skin Microbiome Imbalance in Patients with STAT1/STAT3 Defects Impairs Innate Host Defense Responses
Authors:
Sanne P. Smeekens, c Curtis Huttenhower, Anca Riza, Frank L. van de Veerdonk, Patrick L.J.M. Zeeuwen, Joost Schalkwijk, Jos W.M. van der Meer, Ramnik J. Xavier, Mihai G. Netea, Dirk Gevers
Info:
Genomic DNA was subjected to 16S PCR amplifications. Pippin Prep was used to retrieve the amplicons in order to isolate fragments away from aspecific products, prior to Illumina MiSeq sequencing.
Citation:
J Innate Immun
A Filtering Method to Generate High Quality Short Reads Using Illumina Paired-End Technology
June 2013
Authors:
A. Murat Eren, Joseph H. Vineis., Hilary G. Morrison, Mitchell L. Sogin
Info:
The authors present a method of library preparation for overlapping paired-end sequencing on the Illumina platform, and improved analysis software, to reduce the error in metagenomic studies using NGS of 16S rRNA gene amplicons. The Pippin Prep was used for DNA size selection.
Citation:
PLoS ONE 8(6): e66643
Deciphering the Growth Behaviour of Mycobacterium africanum
May 2013
Authors:
Florian Gehre, Jacob Otu, Kathryn DeRiemer, Paola Florez de Sessions, Martin L. Hibberd, Wim Mulders, Tumani Corrah, Bouke C. de Jong, Martin Antonio
Info:
The Pippin Prep was used to size select libraries prepared with the New England Biolabs NEB Next DNA sample prep kit. DNA was sequenced on the Illumina HiSeq 2000.
Citation:
PLoS Negl Trop Dis 7(5): e2220
Multiplex target capture with double-stranded DNA probes
May 2013
Authors:
Peidong Shen, Wenyi Wang, Aung-Kyaw Chi, Yu Fan, Ronald W Davis, Curt Scharfe
Info:
The authors describe a new method for targeted NGS using double-stranded probes rather than single-stranded ones, improving sensitivity for variant detection, and at a lower cost. The probes consist of single-stranded complimentary long padlock probes (CLPPs), each of which selectively captures one strand of a genomic target through circularization. The Pippin Prep was used to size select libraries for the Illumina MiSeq platform.
Citation:
Genome Medicine 2013, 5:50
RESTseq – Efficient Benchtop Population Genomics with RESTriction Fragment SEQuencing
May 2013
Authors:
Eckart Stolle1, Robin F. A. Moritz
Info:
The authors present a new method for SNP analysis in population genomics. The Pippin Prep was used to size select DNA for the Ion Torrent PGM platform .
Citation:
PLoS ONE 8(5): e63960