Shining a Light on Dark Sequencing: Characterising Errors in Ion Torrent PGM Data
April 2013
Authors:
Lauren M. Bragg, Glenn Stone, Margaret K. Butler, Philip Hugenholtz, Gene W. Tyson
Info:
The authors use the Pippin Prep as an alternative to gel purification for isolating PCR fragments. The authors note that manual gel purification can lead to underrepresentation of low G+C% sequences.
Citation:
PLoS Comput Biol 9(4):e1003031
Transcriptome Analysis of Thapsia laciniata Rouy Provides Insights into Terpenoid Biosynthesis and Diversity in Apiaceae
April 2013
Authors:
Damian Paul Drew, Bjørn Dueholm, Corinna Weitzel, Ye Zhang, Christoph W. Sensen, and Henrik Toft Simonsen
Info:
The Pippin Prep was used to size-select Illumina RNA-seq libraries.
Citation:
Int. J. Mol. Sci. 2013, 14, 9080-9098
Selective Inhibition of Tumor Oncogenes by Disruption of Super-Enhancers
April 2013
Authors:
Jakob Love, Heather A. Hoke, Charles Y. Lin, Ashley Lau, David A. Orlando, Christopher R. Vakoc, James E. Bradner, Tong Ihn Lee, and Richard A. Young
Info:
The Pippin Prep was used to size-select ChIP-seq libraries.
Citation:
Cell 153:320-334 April 2013
H2-Independent Growth of the Hydrogenotrophic Methanogen Methanococcus maripaludis
March/April 2013
Authors:
Kyle C. Costa, Thomas J. Lie, Michael A. Jacobs, John A. Leigh
Info:
The authors constructed deletions in several key metabolism genes to study the mechanism of H2 independent growth in this hydrogenotropic strain. Several mutants were found that shed new light on alternative mechanisms of methanogenic growth. The mutants were mapped by whole genome sequencing, in which Pippin Prep was used to size select the libraries.
Citation:
mBio (4)2:00062-13 March/April 2013
Draft Genome Sequence of Rhodococcus opacus Strain M213 Shows a Diverse Catabolic Potential
January/February 2013
Authors:
Ashish Pathak, Stefan J. Green, Andrew Ogram, Ashvini Chauhana
Info:
Rhodococcus strains have diverse and unusual metabolic pathways that are being considered for industrial roles in bioproduction of chemicals and fuels. The authors present the whole genome sequence of one such strain. Nextera chemistry was used with size selection on the Pippin Prep to generate the sequencing libraries.
Citation:
genomeA (1)1:e00144-12 January/February 2013