Deciphering the Growth Behaviour of Mycobacterium africanum
May 2013
Authors:
Florian Gehre, Jacob Otu, Kathryn DeRiemer, Paola Florez de Sessions, Martin L. Hibberd, Wim Mulders, Tumani Corrah, Bouke C. de Jong, Martin Antonio
Info:
The Pippin Prep was used to size select libraries prepared with the New England Biolabs NEB Next DNA sample prep kit. DNA was sequenced on the Illumina HiSeq 2000.
Citation:
PLoS Negl Trop Dis 7(5): e2220
Multiplex target capture with double-stranded DNA probes
May 2013
Authors:
Peidong Shen, Wenyi Wang, Aung-Kyaw Chi, Yu Fan, Ronald W Davis, Curt Scharfe
Info:
The authors describe a new method for targeted NGS using double-stranded probes rather than single-stranded ones, improving sensitivity for variant detection, and at a lower cost. The probes consist of single-stranded complimentary long padlock probes (CLPPs), each of which selectively captures one strand of a genomic target through circularization. The Pippin Prep was used to size select libraries for the Illumina MiSeq platform.
Citation:
Genome Medicine 2013, 5:50
RESTseq – Efficient Benchtop Population Genomics with RESTriction Fragment SEQuencing
May 2013
Authors:
Eckart Stolle1, Robin F. A. Moritz
Info:
The authors present a new method for SNP analysis in population genomics. The Pippin Prep was used to size select DNA for the Ion Torrent PGM platform .
Citation:
PLoS ONE 8(5): e63960
FOXO1 regulates expression of a microRNA cluster on X chromosome
May 2013
Authors:
Ruchi Singhal, Jonathan E. Bard, Norma J. Nowak, Michael J.Buck, and Eugene S. Kandel
Info:
The Pippin Prep was used in size selection with the Illumina TruSeq Small RNA sequencing protocol.
Citation:
AGING 5(5): 347-356
Shining a Light on Dark Sequencing: Characterising Errors in Ion Torrent PGM Data
April 2013
Authors:
Lauren M. Bragg, Glenn Stone, Margaret K. Butler, Philip Hugenholtz, Gene W. Tyson
Info:
The authors use the Pippin Prep as an alternative to gel purification for isolating PCR fragments. The authors note that manual gel purification can lead to underrepresentation of low G+C% sequences.
Citation:
PLoS Comput Biol 9(4):e1003031