Citations

Genetic Basis for the Biosynthesis of the Pharmaceutically Important Class of Epoxyketone Proteasome Inhibitors

October 2013

Authors:
Michelle Schorn, Judith Zettler, Joseph P. Noel, Pieter C. Dorrestein, Bradley S. Moore, and Leonard Kaysser

Info:
Collaborators in the U.S. and Germany report on the genetic pathway for the production of epoxyketone proteasome inhibitors, used as anti-cancer therapeutic agents, in bacterial strains. The Pippin Prep was used to size select DNA prior to Ion Torrent sequencing.

Citation:
ACS Chem. Biol. 2014, 9, 301−309

doi:10.1021/cb400699p

Posted in Citation | Tagged , , , | Comments Off on Genetic Basis for the Biosynthesis of the Pharmaceutically Important Class of Epoxyketone Proteasome Inhibitors

Quick, sensitive and specific detection and evaluation of quantification of minor variants by high-throughput sequencing

October 2013

Authors:
Ross Ka-Kit Leung, Zhi Qiang Dong, Fei Sa, Cheong Meng Chong, Si Wan Lei,c Stephen Kwok-Wing Tsui and Simon Ming-Yuen Lee

Info:
Scientists from the University of Hong Kong and the University of Macau, China, evaluate errors in detecting minor variants and suggest rules for authenticating the variants and improving accuracy of detection. The Pippin Prep was used for library preparation on the Ion Torrent platform.

Citation:
Mol. BioSyst., 2014, 10, 206

doi:10.1039/c3mb70334g

Posted in Citation | Tagged , , , | Comments Off on Quick, sensitive and specific detection and evaluation of quantification of minor variants by high-throughput sequencing

Genomic architecture of ecologically divergent body shape in a pair of sympatric crater lake cichlid fishes

October 2013

Authors:
PAOLO FRANCHINI, CARMELO FRUCIANO, MARIA L. SPREITZER, JULIA C. JONES, KATHRYN R. ELMER, FREDERICO HENNING and AXEL MEYER

Info:
Researchers at the University of Konstanz, Germany, look at the speciation of chichlid fishes using the ddRAD technique. The Pippin Prep was used to size-select prior to Illumina sequencing. The study indicates a few genomic regions that largely contributed to early divergence.

Citation:

Molecular Ecology (2013)

doi:10.1111/mec.12590

Posted in Citation | Tagged , | Comments Off on Genomic architecture of ecologically divergent body shape in a pair of sympatric crater lake cichlid fishes

Improved Protocols for Illumina Sequencing

October 2013

Authors:
Iraad F. Bronner, Michael A. Quail, Daniel J. Turner, and Harold Swerdlow

Info:
A comprehensive overview of the state-of-the state of Illumina sequencing, from the Wellcome Trust Sanger Institute and Oxford Nanopore Technologies. The value of narrow size distribution and Pippin automated size selection is discussed.

Citation:
Current Protocols in Human Genetics 18.2.1-18.2.42, October 2013

http://dx.doi.org/10.1002/0471142905.hg1802s79

Posted in Citation | Tagged , , , , | Comments Off on Improved Protocols for Illumina Sequencing

Next-Generation Sequence Analysis of Cancer Xenograft Models

Authors:
Fernando J. Rossello, Richard W. Tothill, Kara Britt, Kieren D. Marini, Jeanette Falzon, David M. Thomas, Craig D. Peacock, Luigi Marchionni, Jason Li, Samara Bennett, Erwin Tantoso, Tracey Brown, Philip Chan, Luciano G. Martelotto, D. Neil Watkins

Info:
The authors looked at using xenografts as preclinical models for NGS cancer studies. The authors were able to show that mouse specific sequence was easily distinguished from human small cell lung cancer sequences. Pippin Prep size selection was used for whole genome library construction for the Illumina HiSeq.

Citation:
PLoS ONE 8(9): e74432

http://dx.doi.org/10.1371/journal.pone.0074432

Posted in Citation | Tagged , | Comments Off on Next-Generation Sequence Analysis of Cancer Xenograft Models