PacBio-LITS: a large-insert targeted sequencing method for characterization of human disease-associated chromosomal structural variations
March 2015
Authors:
Min Wang, Christine R Beck, Adam C English, Qingchang Meng, Christian Buhay, Yi Han, Harsha V Doddapaneni, Fuli Yu, Eric Boerwinkle, James R Lupski, Donna M Muzny and Richard A Gibbs
Info:
In this BMC Genomics publication, scientists from Baylor College of Medicine report a new method for targeted sequencing of important structural variations in the human genome with the PacBio platform. Using oligo-based DNA capture with Pippin Prep or BluePippin sizing from Sage Science, the researchers demonstrated successful structural variant analysis at high accuracy and low cost.
Citation:
Wang et al. BMC Genomics (2015) 16:214
DOI 10.1186/s12864-015-1370-2
A phylogenomic analysis of turtles
February 2015
Authors:
Nicholas G Crawford, James F Parham, Anna B Sellas, Brant C Faircloth, Travis C Glenn, Theodore J Papenfuss, James B Henderson, Madison H Hansen, W Brian Simison
Info:
In this Editor’s Choice article from the Molecular Phylogenetics and Evolution journal, researchers provide the first genome-scale analysis of turtle phylogeny using sequence data collected from thousands of ultraconserved elements. Through maximum likelihood, Bayesian, and species tree methods, they produced a single resolved phylogeny. BluePippin was used to prepare the libraries for PCR amplification.
Citation:
Molecular Phylogenetics and Evolution
doi:10.1016/j.ympev.2014.10.021
Enhanced Reduced Representation Bisulfite Sequencing for Assessment of DNA Methylation at Base Pair Resolution
February 2015
Authors:
Francine E. Garrett-Bakelman, Caroline K. Sheridan, Thadeous J. Kacmarczyk, Jennifer Ishii, Doron Betel, Alicia Alonso, Christopher E. Mason, Maria E. Figueroa, and Ari M. Melnick
Info:
In this publication in the Journal of Visualized Experiments, scientists at Weill Cornell Medical College and the University of Michigan describe a protocol to map DNA methylation patterns across the genome with next-gen sequencing instead of microarrays. Where input amounts allow, the approach calls for automated DNA size selection using Pippin Prep.
Citation:
J Vis Exp. 2015; (96): 52246.
DOI: 10.3791/52246
Assessing the utility of whole genome amplified DNA for next-generation molecular ecology
February 2015
Authors:
Christopher Blair, C. Ryan Campbell, and Anne D. Yoder
Info:
Scientists at Duke University explored whether whole-genome amplification leads to sequence bias by performing a ddRAD-seq study of grey mouse lemurs using multiple displacement amplification. They conclude that MDA enrichment does not cause systematic bias, opening the doors for studies with low-quantity DNA. The team used Pippin Prep for size selection of the ddRAD-seq library.
Citation:
Molecular Ecology Resources (2015) 15, 1079–1090
doi: 10.1111/1755-0998.12376
PU.1 Opposes IL-7–Dependent Proliferation of Developing B Cells with Involvement of the Direct Target Gene Bruton Tyrosine Kinase
December 2014
Authors:
Darah A. Christie, Li S. Xu, Shereen A. Turkistany, Lauren A. Solomon, Stephen K. H. Li, Edmund Yim, Ian Welch, Gillian I. Bell, David A. Hess, and Rodney P. DeKoter
Info:
Scientists from the University of Western Ontario report in this Journal of Immunology paper a research effort to understand how faulty B cell development in mice leads to a form of leukemia. Ultimately, they determined that inducing Btk expression leads to apoptosis, perhaps preventing leukemia formation. Pippin Prep sizing was used for ChIP-seq on an Illumina HiSeq 2000.
Citation:
doi:10.4049/jimmunol.1401569