MicroRNAs in the oriental fruit fly, Bactrocera dorsalis: extending Drosophilid miRNA conservation to the Tephritidae
October 2015
Authors:
Bernarda Calla and Scott M. Geib
Info:
Scientists analyzed small RNAs in Bactrocera dorsalis, the oriental fruit fly, and compared the resulting gene expression profile with that of Drosophila melanogaster. BluePippin was used to enrich for cDNAs between 92 bp and 118 bp prior to sequencing on an Ion Torrent PGM.
Citation:
BMC Genomics 2015 16:740
DOI: 10.1186/s12864-015-1835-3
Scarless genome editing and stable inducible expression vectors for Geobacter sulfurreducens
August 2015
Authors:
Chi Ho Chan, Caleb E. Levar, Lori Zacharoff, Jonathan P. Badalamenti, Daniel R. Bond
Info:
Scientists from the University of Minnesota report in Applied and Environmental Microbiology the development of genetic system improvements for Geobacter sulfurreducens. BluePippin was used to size-select libraries prior to sequencing on PacBio.
Citation:
doi:10.1128/AEM.01967-15
A multidrug resistance plasmid contains the molecular switch for type VI secretion in Acinetobacter baumannii
July 2015
Authors:
Brent S. Weber, Pek Man Ly, Joshua N. Irwin, Stefan Pukatzki, Mario F. Feldman
Info:
Scientists from the University of Alberta and Washington University published results in PNAS showing that a large plasmid is important to Acinetobacter baumannii’s ability to resist antibiotic treatment. The team prepared 20 Kb libraries for PacBio sequencing, using BluePippin for size selection.
Citation:
PNAS vol. 112 no. 309442-9447
doi:10.1073/pnas.1502966112
Next generation sequencing to identify novel genetic variants causative of autosomal dominant familial hypercholesterolemia associated with increased risk of coronary heart disease
July 2015
Authors:
Faisal A. Al-Allaf, Mohammad Athar, Zainularifeen Abduljaleel, Mohiuddin M. Taher, Wajahatullah Khan, Faisal A. Ba-hammam, Hala Abalkhail, Abdullah Alashwal
Info:
Scientists from Saudi Arabia published a paper in Gene demonstrating the use of targeted sequencing to detect pathogenic variants associated with familial hypercholesterolemia, including the discovery of a novel heterozygous variant. Pippin Prep was used for DNA size selection prior to sequencing on an Ion Torrent PGM.
Citation:
Gene
doi: 10.1016/j.gene.2015.03.064
Isolation via next-generation sequencing of microsatellites from the Tasmanian macroalgae Lessonia corrugata (Lessoniaceae)
July 2015
Authors:
Halley M. S. Durrant, Christopher P. Burridge, and Michael G. Gardner
Info:
Scientists from Australia developed microsatellite markers for this macroalgae, an important component of the local marine ecosystem, that will allow for future population genetic studies of the organism. They used Pippin Prep to size select libraries for Ion Torrent sequencing.
Citation:
Appl Plant Sci. 2015 Jul; 3(7)
doi: 10.3732/apps.1500042