Synthetic Spike-in Standards Improve Run-Specific Systematic Error Analysis for DNA and RNA Sequencing
July 2012
Authors:
Justin M. Zook, Daniel Samarov, Jennifer McDaniel, Shurjo K. Sen, Marc Salit
Info:
New approach to discovery of systematic sequencing errors by use of spike in RNA or DNA control DNAs. Pippin Prep used for RNA-seq library construction. Paired end reads on Illumina GAIIx.
Citation:
PLoS ONE 7(7): e41356.
Double Digest RADseq: An Inexpensive Method for De Novo SNP Discovery and Genotyping in Model and Non-Model Species
May 2012
Authors:
Brant K. Peterson, Jesse N. Weber, Emily H. Kay, Heidi S. Fisher, Hopi E. Hoekstra
Info:
An NGS method that allows inexpensive HT genotyping at moderate depth without prior knowledge of any markers. A high impact paper. Extremely reproducible size selection is crucial to the method and the authors used Pippin Prep for this purpose.
Citation:
PLoS ONE 7(5): e37135. doi:10.1371/journal.pone.0037135
Low Incidence of DNA Sequence Variation in Human Induced Pluripotent Stem Cells Generated by Nonintegrating Plasmid Expression
March 2012
Authors:
Linzhao Cheng, Nancy F. Hansen, Ling Zhao, Yutao Du, Chunlin Zou, Frank X. Donovan, Bin-Kuan Chou, Guangyu Zhou, Shijie Li, Sarah N. Dowey, Zhaohui Ye, NISC Comparative Sequencing Program,
Settara C. Chandrasekharappa, Huanming Yang, James C. Mullikin, and P. Paul Liu
Info:
Whole genome sequencing on reprogrammed stem cell lines. Materials and Methods: Covaris shearing followed by size selection at 450 bp on the Pippin Prep. Illumina TruSeq chemistry on HiSeq 2000, 90 or 101 bp paired end protocols.
Citation:
Cell Stem Cell 10, 337–344, March 2, 2012
Mutation discovery in mice by whole exome sequencing
December 2011
Authors:
Heather Fairfield, Griffith J Gilbert, Mary Barter, Rebecca R Corrigan, Michelle Curtain, Yueming Ding, Mark D’Ascenzo, Daniel J Gerhardt, Chao He, Wenhui Huang, Todd Richmond, Lucy Rowe, Frank J Probst,
David E Bergstrom, Stephen A Murray, Carol Bult, Joel Richardson, Benjamin T Kilew, Ivo Gut, Jorg Hager, Snaevar Sigurdsson, Evan Mauceli, Federica Di Palma, Kerstin Lindblad-Toh, Michael L Cunningham,
Timothy C Cox, Monica J Justice, Mona S Spector, Scott W Lowe, Thomas Albert, Leah Rae Donahue, Jeffrey Jeddeloh, Jay Shendure and Laura G Reinholdt
Info:
Materials and Methods: Illumina PE libraries were constructed and size selected at 430bp “tight” setting on Pippin Prep prior to enrichment PCR and hybridization capture for whole exome regions.
Citation:
Genome Biology 2011, 12:R86
Molecular characterization of the translocation breakpoints in the Down syndrome mouse model Ts65Dn
September 2011
Authors:
Laura G. Reinholdt, Yueming Ding, Griffith T. Gilbert, Anne Czechanski, Jeffrey P. Solzak, Randall J. Roper, Mark T. Johnson, Leah Rae Donahue, Cathleen Lutz, Muriel T. Davisson
Info:
Illumina PE libraries were constructed and size selected at 400bp “tight” setting on Pippin Prep prior to enrichment PCR and hybridization capture for genomic region surrounding the Down syndrome locus in mouse, to identify rearrangements in a much-studied Down’s syndrome model strain.
Citation:
Mamm Genome (2011) 22:685–691