Identification of Human Islet Amyloid Polypeptide as a BACE2 Substrate
February 2016
Authors:
Ingrid C. Rulifson, Ping Cao, Li Miao, David Kopecky, Linda Huang, Ryan D. White, Kim Samayoa, Jonitha Gardner, Xiaosu Wu, Kui Chen, Trace Tsuruda, Oliver Homann, Helene Baribault, Harvey Yamane, Tim Carlson, Jed Wiltzius, Yang Li
Info:
Researchers from Amgen report on a proteolytic enzyme, BACE2, that could potentially have a therapeutic use for treatment of pancreatic amyloid formation caused by Type-2 diabetes. Illumina RNA-seq, with Pippin size selection, was used to test whether BACE would modulate its target proteins in a cellular setting.
Citation:
PLoS ONE 11(2): e0147254
doi:10.1371/journal.pone.0147254
Pitfalls of haplotype phasing from amplicon-based long-read sequencing
February 2016
Authors:
Thomas W. Laver, Richard C. Caswell, Karen A. Moore, Jeremie Poschmann, Matthew B. Johnson, Martina M. Owens, Sian Ellard, Konrad H. Paszkiewicz & Michael N. Weedon
Info:
University of Exeter researchers used long-range PCR and long-read sequencing to phase genetic variants separated by 9 Kb, comparing results from the PacBio and Oxford Nanopore sequencing systems. BluePippin was used for size selection of the PacBio 20 Kb library.
Citation:
Scientific Reports 6, Article number: 21746 (2016)
doi:10.1038/srep21746
Comparison of Sample Preparation Methods Used for the Next-Generation Sequencing of Mycobacterium tuberculosis
February 2016
Authors:
Andrea D. Tyler, Sara Christianson, Natalie C. Knox, Philip Mabon, Joyce Wolfe, Gary Van Domselaar, Morag R. Graham, and Meenu K. Sharma
Info:
In this PLoS One publication, scientists in Manitoba compare the quality of libraries constructed with Illumina’s Nextera and TruSeq protocols for Mycobacterium tuberculosis, which has a particularly challenging genome. They determined that the TruSeq method yielded more uniform data quality and coverage. BluePippin was used for size selection prior to sequencing on a MiSeq.
Citation:
DOI: 10.1371/journal.pone.0148676
The Many Dimensions of Diet Breadth: Phytochemical, Genetic, Behavioral, and Physiological Perspectives on the Interaction between a Native Herbivore and an Exotic Host
February 2016
Authors:
Joshua G. Harrison, Zachariah Gompert, James A. Fordyce, C. Alex Buerkle, Rachel Grinstead, Joshua P. Jahner, Scott Mikel, Christopher C. Nice, Aldrin Santamaria, and Matthew L. Forister
Info:
In this study, scientists analyzed butterflies colonizing several different populations of alfalfa, finding significant variation in larval performance between plant groups. Plant DNA was sequenced on an Illumina HiSeq after size selection of library fragments with BluePippin.
Citation:
PLoS One
DOI: 10.1371/journal.pone.0147971
Experimental feline enteric coronavirus infection reveals an aberrant infection pattern and shedding of mutants with impaired infectivity in enterocyte cultures
January 2016
Authors:
Lowiese M. B. Desmarets, Ben L. Vermeulen, Sebastiaan Theuns, Nádia Conceição-Neto, Mark Zeller, Inge D. M. Roukaerts, Delphine D. Acar, Dominique A. J. Olyslaegers, Marc Van Ranst, Jelle Matthijnssens, and Hans J. Nauwyncka
Info:
Scientists in Belgium studied fecal samples from three cats to understand evolutionary mutations in the feline enteric coronavirus genome, which can cause feline infectious peritonitis. DNA libraries were size-selected with BluePippin prior to sequencing on an Illumina HiSeq.
Citation:
Sci Rep. 2016; 6: 20022