The RNA Exosome Syncs IAV-RNAPII Transcription to Promote Viral Ribogenesis and Infectivity
April 2017
Authors:
Alexander Rialdi, Judd Hultquist, David Jimenez-Morales, Zuleyma Peralta, et al.
Info:
Scientists in New York and California teamed up to study the role of nuclear RNA exosomes in viral transcription, using influenza A. They found that the exosome coordinates transcription and that viral growth is suppressed when the RNA exosome’s activity is reduced. In the Illumina sequencing workflow, BluePippin was used to size-select PCR products.
Citation:
Cell
Impaired human immunodeficiency virus type 1 replicative fitness in atypical viremic non-progressor individuals
March 2017
Authors:
Jan Weber, Richard Gibson, Lenka Sácká, Dmytro Strunin, et al.
Info:
In this publication, scientists describe an analysis of the rates of HIV progression in several patients. They determined that reduced viral replicative fitness appears to be associated with patients whose infections do not progress to advanced AIDS. Deep sequencing of the HIV-1 genome was performed on the Ion PGM after size-selection with Pippin Prep.
Citation:
AIDS Res Ther (2017) 14:15
DOI 10.1186/s12981-017-0144-0
Functional variation in allelic methylomes underscores a strong genetic contribution and reveals novel epigenetic alterations in the human epigenome
March 2017
Authors:
Warren Cheung, Xiaojian Shao, Andréanne Morin, Valérie Siroux, et al.
Info:
Researchers at McGill University and collaborating institutions used ChIP-seq to elucidate the effects of epigenetics and phenotype across the human genome. They analyzed more than 900 samples to understand the effects of epigenetic regulation. The team used Pippin Prep to size-select fragments prior to sequencing on an Illumina HiSeq 2000.
Citation:
Genome Biology (2017) 18:50
DOI 10.1186/s13059-017-1173-7
Defining the diverse spectrum of inversions, complex structural variation, and chromothripsis in the morbid human genome
March 2017
Authors:
Ryan Collins, Harrison Brand, Claire Redin, Carrie Hanscom, et al.
Info:
Scientists at Harvard and other institutions sequenced nearly 700 individuals with autism spectrum disorder to build a comprehensive map of large structural variants. More than a third of the variants detected are novel. BluePippin was used to size-select libraries prior to library construction for 10x Genomics analysis.
Citation:
Genome Biology (2017) 18:36
DOI 10.1186/s13059-017-1158-6
Asymmetric subgenome selection and cis-regulatory divergence during cotton domestication
March 2017
Authors:
Maojun Wang, Lili Tu, Min Lin, Zhongxu Lin, et al.
Info:
Scientists from China and the UK scanned the genomes of 352 varieties of cotton, both wild and domestic, to map variants associated with crop domestication throughout its history. They identified 19 candidate variants that may be related to fiber quality. For this project, the team used PippinHT to isolate purified DNA fragments prior to sequencing with an Illumina HiSeq 2000.
Citation:
Nature Genetics 49, 579–587 (2017)
doi:10.1038/ng.3807