Long-read sequencing and de novo assembly of a Chinese genome
June 2016
Authors:
Lingling Shi, Yunfei Guo, Chengliang Dong, John Huddleston, Hui Yang, et al.
Info:
Scientists in China and the US analyzed the DNA and RNA of a Chinese individual, using PacBio sequencing and BioNano Genomics maps to produce a high-quality assembly. They report a significant amount of new sequence data and novel gene content, including elements that appear to be specific to the Asian population. The team used BluePippin for size selection prior to genome and transcriptome sequencing with PacBio.
Citation:
Nature Communications
doi:10.1038/ncomms12065
Extensive sequencing of seven human genomes to characterize benchmark reference materials
June 2016
Authors:
Justin M. Zook, David Catoe, Jennifer McDaniel, Lindsay Vang, et al.
Info:
The Genome in a Bottle Consortium used 12 different DNA analysis technologies to generate deep data sets on seven genomes, five of which will become reference materials for the community. BluePippin was used with various platforms, including PacBio and SOLiD.
Citation:
Scientific Data 2016; 3: 160025.
DOI 10.1038/sdata.2016.25
A highly robust and optimized sequence-based approach for genetic polymorphism discovery and genotyping in large plant populations
May 2016
Authors:
Ning Jiang, Fengjun Zhang, Jinhua Wu, Yue Chen, Xiaohua Hu, Ou Fang, Lindsey J. Leach, Di Wang, Zewei Luo
Info:
Scientists in China and the UK developed an optimized RAD-seq protocol that delivers better uniformity of genome coverage. The workflow involves two Pippin Prep size selections.
Citation:
Theor Appl Genet
DOI 10.1007/s00122-016-2736-9
Multi-omics maps of cotton fibre reveal epigenetic basis for staged single-cell differentiation
April 2016
Authors:
Maojun Wang, Pengcheng Wang, Lili Tu, Sitao Zhu, Lin Zhang, Zhonghua Li, Qinghua Zhang, Daojun Yuan, and Xianlong Zhang
Info:
Scientists from Huazhong Agricultural University in China studied the epigenetics of single-cell differentiation
in cotton, finding clear patterns of DNA methylation during development. They used PippinHT to isolate 100 bp – 200 bp fragments which were run in MNase-seq and ChIP-seq pipelines on an Illumina HiSeq 3000.
Citation:
Nucleic Acids Research, 2016
doi: 10.1093/nar/gkw238
Urbanization shapes the demographic history of a native rodent (the white-footed mouse, Peromyscus leucopus) in New York City
April 2016
Authors:
Stephen E. Harris, Alexander T. Xue, Diego Alvarado-Serrano, Joel T. Boehm, Tyler Joseph, Michael J. Hickerson, Jason Munshi-South
Info:
Researchers at the City University of New York studied populations of white-footed mice in New York City and surrounding areas, discovering that urbanization and climate change shaped population diversity and evolution of these animals. The team used ddRAD-seq with Pippin Prep for automated DNA size selection.
Citation:
Biology Letters
DOI: 10.1098/rsbl.2015.0983