BEVERLY, Mass. — February 12, 2014 — Sage Science, a developer of products for improving sample preparation processes in life science applications, today announced the release of its new SageELF technology for DNA and protein fractionation. This sample prep instrument will be especially useful for preserving precious samples and has applications in next-generation sequencing as well as bottom-up or top-down proteomics experiments.
The SageELF generates 12 contiguous fractions from whole DNA or protein samples. For DNA or cDNA sequencing, that allows scientists to generate libraries with multiple insert sizes from the same sample, which has applications in genome sequencing, structural variant detection, and identification of rare splice variants. For protein studies, the ability to take many slices from a whole sample is useful for reducing sample complexity, enriching for rare proteins, and ensuring recovery of all proteins in the sample.
“As the leading provider of automated products for targeted DNA size selection, we are pleased to offer this new tool for whole-sample fractionation. We believe that users will find the SageELF to be more reproducible and precise than manual gels or columns,” said Todd Barbera, the COO of Sage Science. “The ability to maximize value from every single sample is in line with our commitment to the sample prep market, where we target labor-intensive steps that can be significantly improved with accurate, automated solutions.”
The SageELF, short for Electrophoretic Lateral Fractionator, automatically electrophoreses samples and elutes whole DNA or proteins from the entire gel lane into 12 elution modules. Users simply load their sample, start the run, and pipette out the fractions later. Unlike in-gel digestion, in which some DNA fragments or peptides never leave the gel, the whole protein or DNA sample is electrophoresed into the elution modules.
Sage Science also manufactures the Pippin line of automated tools for targeted DNA size selection. Independent studies of size-selection methods for next-generation sequencing sample prep have repeatedly found that the Pippin platform offers unparalleled reproducibility, accuracy, and sample recovery. Precise size selection is critical for optimizing sequencing efficiency, improving genome assemblies, and reducing project costs.
To learn more about the SageELF, visit www.sagescience.com/sage-elf
BEVERLY, Mass. AND SAO PAULO — June 25, 2013 — Sage Science, a developer of products for improving sample preparation processes in life science applications, and Uniscience do Brasil, the leading distributor of equipment and reagents to the Brazilian biotechnology and molecular biology markets, today announced a distribution agreement for Sage’s Pippin DNA size selection product portfolio. Uniscience is the first distributor for Sage Science in the South American market.
“Uniscience is very pleased to be working with Sage Science and we are looking forward to collaborating with them to open up the Brazilian scientific market to their automated DNA size selection technologies,” said Sergio Joelsons, CEO and President of Uniscience. “Next-generation sequencing is a rapidly growing segment of the research market in South America and through this agreement we are taking another step toward offering our customers a complete line of innovative workflow solutions for DNA analysis.”
The Pippin DNA size selection system consists of an electrophoresis instrument and pre-cast, disposable gel cassettes. For collections of smaller fragments up to 1.5 kb, the Pippin Prep system facilitates the construction of next-generation sequencing libraries for paired-end sequencing, ChIP-seq, and preparing templates for emulsion PCR. If the collection of larger fractions is needed, the BluePippin system allows researchers to expand size selection to fragments up to 50 kb using pulsed-field electrophoresis. Pippin systems can be used as a replacement for manual gel purification for any application that requires, or can be improved by, this step.
“We are thrilled to be working with Uniscience as our first distributor in South America,” said Gary Magnant, CEO of Sage Science. “We believe the Pippin product portfolio provides the de facto standard for tightly sized libraries with the highest quality in next-generation DNA sequencing, and we look forward to supporting the Brazilian research community in team with Uniscience.”
Independent studies of size-selection methods for next-generation sequencing sample prep have repeatedly found that the Pippin platform offers unparalleled reproducibility, accuracy, and sample recovery. Precise size selection is critical for optimizing sequencing efficiency, improving genome assemblies, and reducing project costs.
For a complete list of Pippin distributors worldwide, please visit: www.sagescience.com/about-us/distributors.
About Uniscience do Brasil
Founded in 1984, Uniscience do Brasil is the most complete Biotechnology distributor in the Brazilian market. Uniscience brings to the Brazilian market a range of state-of-the-art products made by world renowned manufacturers. Based in Sao Paulo, Uniscience covers the entire Brazilian market with its highly skilled team and specialized distributors network. Uniscience’s commitment to its customers and business partners is reflected in the high quality of products and services it provides. For more information, please visit http://www.uniscience.com.
MENLO PARK, Calif. and BEVERLY, Mass. — May 16, 2013 — Pacific Biosciences of California, Inc., (NASDAQ:PACB), provider of the PacBio® RS II DNA Sequencing System, and Sage Science, a developer of life science products for improving sample preparation processes in molecular biology applications, today announced a co-marketing partnership to provide customers of the PacBio RS II the ability to sequence even longer DNA fragments when performing Single Molecule, Real-Time (SMRT®) Sequencing.
“We have found that the BluePippin™ size selection platform is very useful for applications where long read lengths are important, such as de novo genome assembly,” said Kevin Corcoran, Senior Vice President of Market Development at Pacific Biosciences. “Our studies have demonstrated that size selection with Sage’s BluePippin platform is an easy addition to our customers’ sample prep workflow that selects for 10 kb or larger fragments for sequencing on the PacBio RS II.”
Based on PacBio’s revolutionary SMRTSequencing technology, the PacBio RS II can generate average read lengths of 5,000 base pairs, with the longest reads above 20,000 base pairs in length. The PacBio RS II System, including consumables and software, provides a simple, fast, end-to-end workflow for SMRT Sequencing in research areas including infectious disease and microbiology, agriculture, and complex genetic diseases with repeat expansions.
The BluePippin platform, part of the Pippin line of automated size-selection tools offered by Sage Science, uses pulsed-field power to take fractions of DNA from fragments ranging from 50 bases to 50 kilobases. Independent studies of size-selection methods for next-generation sequencing sample prep have repeatedly found that the Pippin platform offers unparalleled reproducibility, accuracy, and sample recovery. By eliminating low-molecular-weight templates from sequencing libraries, automated size selection allows users to load longer fragments and boost the average read length produced by sequencing.
“This is excellent validation of the tremendous utility BluePippin offers for libraries designed to generate extraordinarily long sequence reads,” said Alex Vira, Director of Marketing for Sage Science. “We look forward to working with PacBio and its customers as they continue to deliver industry-leading read lengths and conduct countless studies that would be impossible with shorter-read technologies.”
In data presented at the Advances in Genome Biology & Technology conference earlier this year, Pacific Biosciences scientists assessed three libraries (bacterial, fish and mammalian) generated with and without BluePippin size selection. They reported that N50 subread lengths increased by 90%, 110%, and 86%, respectively, in the libraries that were size selected with BluePippin compared to those that were not.
About Pacific Biosciences
Pacific Biosciences of California, Inc. (Nasdaq:PACB) offers the PacBio® RS II DNA Sequencing System to help scientists solve genetically complex problems. Based on its novel Single Molecule, Real-Time (SMRT®) technology, the company’s products enable: targeted sequencing to more comprehensively characterize genetic variations; de novo genome assembly to more fully identify, annotate and decipher genomic structures; and DNA base modification identification to help characterize epigenetic regulation and DNA damage. By providing access to information that was previously inaccessible, Pacific Biosciences enables scientists to increase their understanding of biological systems. More information is available at www.pacb.com.
BEVERLY, Mass. — January 14, 2013 — Sage Science, a developer of life science products for improving sample preparation processes in molecular biology applications, today announced the launch of Pippin Link and Pippin Pulse, two new products designed to streamline library prep workflow and enhance the utility of the Pippin line of automated DNA size selection products.
The Pippin Link is an accessory for the Pippin Prep and BluePippin that lets users boost throughput by connecting up to eight instruments and controlling them from a single computer. This streamlines workflow and conserves bench space. The Pippin Pulse is a pulsed-field electrophoresis power supply for analytical gels. Based on the power supply of the BluePippin, Pippin Pulse is an affordable tool that lets researchers resolve DNA to 100 Kb and beyond. It uses a simple software interface that provides users full flexibility in developing their own field-inversion protocols.
“The trend we have seen is that scientists are buying multiple Pippin size selection instruments for their labs, so we developed the Pippin Link specifically for those customers to keep the process as simple and hands-off as possible,” said Chris Boles, Chief Scientific Officer of Sage Science. “Our other new release, Pippin Pulse, was designed for researchers who are trying to resolve very large DNA fragments and are looking for a low-cost, straightforward solution.”
Independent studies of size selection methods have repeatedly demonstrated that the Pippin platform offers unparalleled reproducibility, accuracy, and sample recovery. Precise size selection is critical for optimizing sequencing efficiency, improving genome assemblies, and reducing project costs.
“With the launch of these products, we’re pleased to be adding even more flexibility and multiplexing options for our customers,” said Alex Vira, Director of Marketing for Sage Science. “Precise size selection is a fundamental element for a successful sequencing project, and these new tools will improve researchers’ ability to get the best results with a more efficient workflow.”
BEVERLY, Mass. February 1, 2012 — Sage Science, Inc. today announced the introduction of the BluePippin, an instrument that automates the size selection and retrieval of DNA for molecular biology research. The BluePippin expands Sage’s Pippin line of preparative electrophoresis products by providing the capability to collect larger, kilobase-sized, DNA fragments. The Pippin platform is typically used to construct genomic libraries for next-gen sequencing.
The BluePippin is an updated version of Sage’s Pippin Prep system, to which a pulsed-field power supply has been added. Pulsed field gel electrophoresis allows larger fragments of DNA separated with high resolution and accuracy. Using pre-cast gels, the BluePippin can automatically collect DNA targets up to 50 kilobase pairs, with minimal hands-on time. The system will also feature a new optical design that improves the automated capture of DNA bands and restriction fragments.
Sage Science’s CEO, Gary Paul Magnant, commented on the BluePippin. “ We are pleased to be providing researchers an even more flexible alternative to a difficult manual procedure. The BluePippin pulsed-field feature should help enable next-gen sequencing techniques such as mate-pair sequencing, and address third generation sequencing where the requirement of intact high molecular weight DNA is critical. We also expect that our upper range of size selection will continue to increase with development, which should address an ever broader range of laboratory applications.”