Ting Hon, Kristin Mars, Greg Young, Yu-Chih Tsai, Joseph W. Karalius, Jane M. Landolin, Nicholas Maurer, David Kudrna, Michael A. Hardigan, Cynthia C. Steiner, Steven J. Knapp, Doreen Ware, Beth Shapiro, Paul Peluso, David R. Rank
Pacific Biosciences, in conjunction with their research partners, publicly released (in a BioRxiv preprint) the sequences of five complex genomes that were derived using their high-accuracy circular consensus sequencing (the HiFi method, which resequences 15-20kb SMRTbells up to 30X). The genomes include inbred models Mus musculus (house mouse) and Zea mays,(corn) and complex models Fragaria × ananassa (Strawberries) and Rana muscosa (mountain yellow-legged frog). The fifth sequence was a mock metagenomic bacterial community (ATCC MSA-1003).
Three of the libraries use the SageELF for narrow size selections at ~15KB. The BluePippin was used for one genome (beads were used to size select the metagenomic samples.
Pacific Biosciences, Menlo Park CA
Ravel Biotechnology, San Francisco CA
University of California Santa Cruz, Santa Cruz CA
University of Arizona, Tuscon AR
University of California Davis, Davis CA
Cold Spring Harbor Laboratory, Cold Spring Harbor, NY
USDA-ARS, Ithaca NY
Howard Hughes, Medical Institute, University of California Santa Cruz
Giorgia G. Auteri & L. Lacey Knowles
Little brown bats have experienced a 90% population decline due to fungal pathogen that causes a disease, white-nose syndrome. The authors used ddRADseq to compare surviving and non-surviving individuals. The results indicate shifts in allelic frequencies in genes associated with responses to the disease, suggesting evolutionary adaptation to the syndrome.
Pippin Prep was used to size-select ddRad libraries.
Department of Ecology and Evolutionary Biology and Museum of Zoology, University of Michigan, Ann Arbor, MI
Nature Scientific Reports
A restriction enzyme reduced representation sequencing approach for low-cost, high-throughput metagenome profiling
Melanie K. Hess, Suzanne J. Rowe, Tracey C. Van Stijn, Hannah M. Henry, Sharon M. Hickey, Rudiger Brauning, Alan F. McCulloch, Andrew S. Hess, Michelle R. Kirk, Sandeep Kumar, Cesar Pinares-Patiño, Sandra Kittelmann, Graham R. Wood, Peter H. Janssen, John C. McEwan
The authors present a restriction enzyme reduced representation sequencing (RE-RRS) method for metagenome profiling of livestock rumen microbial communities. The researchers correlate the profiles with methane production from the animals and compare results to previous 16S sequencing benchmarks.
Pippin Prep was used to size-select amplified sequences.
AgResearch Limited, Invermay Agricultural Centre, Mosgiel, New Zealand
AgResearch Limited, Ruakura Agricultural Centre, Hamilton, New Zealand
AgResearch Limited, Grasslands Research Centre, Palmerston North, New Zealand
Phuong-Uyen C. Dinh, Dipti Paudel, Hayden Brochu, Kristen D. Popowski, M. Cyndell Gracieux, Jhon Cores, Ke Huang, M. Taylor Hensley, Erin Harrell, Adam C. Vandergriff, Arianna K. George, Raina T. Barrio, Shiqi Hu, Tyler A. Allen, Kevin Blackburn, Thomas G. Caranasos, Xinxia Peng, Lauren V. Schnabel, Kenneth B. Adler, Leonard J. Lobo, Michael B. Goshe & Ke Cheng
Collaborators at North Carolina State University report success treating Idiopathic pulmonary fibrosis (IPF) with inhalation treatment with lung spheroid cell-secretome and exosomes using mouse models. IPF is a fatal and incurable form of interstitial lung disease in which persistent injury results in scar tissue formation.
Pippin Prep was used to isolate microRNA sequencing libraries for studying the microRNA profile of exosomes post-treatment.
North Carolina State University, Raleigh NC
A spontaneous complex structural variant in rcan-1 increases exploratory behavior and laboratory fitness of Caenorhabditis elegans
Yuehui Zhao, Lijiang Long, Jason Wan, Shweta Biliya, Shannon C. Brady, Daehan Lee, Akinade Ojemakinde, Erik C. Andersen, Fredrik O. Vannberg, Hang Lu, Patrick T. McGrath
Researchers studying genetic adaptive changes using C. elegans recombinant inbred lines (RIL). A single RIL with a high fitness and exploration phenotype was observed that did not share these traits relative to the parental strains. Short-read sequencing indicated a spontaneous complex rearrangement of the rcan-1 gene. This rearrangement was resolved into five unique tandem inversion/duplications using Oxford Nanopore long-read sequencing. The authors suggest that colonization of new environments can create evolutionary pressure to produce complex rearrangements that can act to modify gene expression in ways besides increased gene dosage.
BluePippin was used to size select 10-50kb DNA fragments for Oxford Nanopore MinION library preparation.
Georgia Institute of Technology, Atlanta, Georgia
Northwestern University, Evanston, Illinois