A genome-wide survey of genetic variation in gorillas using reduced representation sequencing
January 2013
Authors:
Aylwyn Scally, Bryndis Yngvadottir, Yali Xue, Qasim Ayub, Richard Durbin, Chris Tyler-Smith
Info:
The study used reduced representation libraries to study diversity among the two currently recognized species of gorilla. The libraries were generated by AluI digestion followed by size selection on the Pippin Prep for AluI fragments between 150-250 bp.
Citation:
pre-print
Use of microarray hybrid capture and next-generation sequencing to identify the anatomy of a transgene
January 2013
Authors:
Amanda J. DuBose, Stephen T. Lichtenstein, Narisu Narisu, Lori L. Bonnycastle, Amy J. Swift, Peter S. Chines and Francis S. Collins
Info:
The authors designed a general method to study the arrangement of foreign DNA and host DNA rearrangements in trangenic animals. The protocol involves standard Illumina PE library generation, using the Pippin Prep for size selection, followed by microarray hybridization capture to isolate fragments containing the transgene.
Citation:
Nucleic Acids Research, 2013, 1-5
Discrete genetic modules are responsible for complex burrow evolution in Peromyscus mice
January 2013
Authors:
Jesse N. Weber, Brant K. Peterson & Hopi E. Hoekstra
Info:
The work seeks to identify genetic influences on a complex behavior in mice, burrow formation. The authors used a modified RADseq procedure to genotype two species of wild mice with distinct burrowing behavior. Pippin Prep was used for size selection in their RADseq protocol.
Citation:
Letter, Nature 493:402-405
A Hybrid Genetic Linkage Map of Two Ecologically and Morphologically Divergent Midas Cichlid Fishes(Amphilophus spp.) Obtained by Massively Parallel DNA Sequencing (ddRADSeq)
January 2013
Authors:
Hans Recknagel, Kathryn R. Elmer, and Axel Meyer
Info:
RADseq was used to develop linkage maps for two highly divergent Cichlid fish. Pippin Prep was used to size select the libraries.
Citation:
G3 (Bethesda) 3(1):65-74 January 2013
IL-10 Produced by Induced Regulatory T Cells (iTregs) Controls Colitis and Pathogenic Ex-iTregs during Immunotherapy
December 2012
Authors:
Erica G. Schmitt, Dipica Haribhai, Jason B. Williams, Praful Aggarwal, Shuang Jia, Louis-Marie Charbonnier, Ke Yan, Rachel Lorier, et al.
Info:
The study included sequencing of the T-cell receptor. For this sequencing, the authors used Ion Torrent platform. The Pippin Prep was used for size-selection as per the standard Life/Ion library construction protocols.
Citation:
J Immunology 189(12): 5638-5648: December 2012