BluePippin

Benefits of the BluePippin System

• Saves time and effort compared to manual gel purification
• Sample-to-sample reproducibility streamlines research
• Higher sample quality improves downstream analysis

Key Downstream Technologies

• Mate-pair sequencing
• Single molecule sequencing
• Bar-coded linked reads

Pre-Cast Agarose Gel Cassettes for DNA Size Selection

Gel cassettes for DNA selections are available in four agarose concentrations to provide a full range of size selection options between 100bp up to 50kb.  For smaller fragment sizes, internal standards may be used for higher accuracy and increased sample capacity (from 4 to 5 samples/cassette).  Higher molecular weight fragments require a dedicated lane to run a calibration marker.  Run times are as little as 30 minutes,  To resolve very large DNA fragments, pulsed-field runs may last several hours. View the BluePippin cassette reference chart to explore the DNA size selection options and run times.

Standard DNA Size Selection Cassettes

• 3% agarose, 100-250bp
• 2% agarose, 100-600bp
• 1.5% agarose, 250bp – 1.5kb
• 0.75% agarose, 1-50kb
• 0.75% agarose, >4-40kb High-Pass
• 0.75% agarose, “Range+T” 9-30kb Size Selection

See the Workflow Summary.

High Pass Plus Cassettes

Faster run times and higher (10ug) input capacity

• >15KB and >20kb High Pass

Learn more here.

Downstream Technologies

• miRNA isolation
• DNA sequencing, RNA-seq, genotyping
• DNA sequencing, ChIP-seq
• Long-read sequencing (PacBio, Oxford Nanopore)