miRNA Library Isolation

[wptabs] [wptabtitle]Description[/wptabtitle] [wptabcontent]
Automated Removal of Adapter Dimers

The Pippin 3% agarose gel cassette was developed for the preparation of miRNA libraries. Adapter-ligated cDNAs of micro RNAs (typically between 100-170 base pairs depending on the adapter size) can be efficiently isolated away from neighboring adapter dimers and larger non-miRNA species (click the thumbnail to enlarge).

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[wptabtitle]Resources[/wptabtitle] [wptabcontent]

• Brochure and Datasheets
  
• Whitepapers
  
  • Sage Pippin: Say Goodbye to Manual Gels
• Posters and Presentations
  
  • ABRF 2013: Sage - " New Automated Systems for Size-Fractionation of Protein Samples"
  • AGBT 2013: Sage - "Introducing the Sage ELF: Multiple DNA size fractions from the same sample"
  • AGBT 2013: Epicentre- "Gene, Transcription Start Site and Splice Site Identification Increased Through Use of Automated Preparative Gel Electrophoresis"
  • AGBT 2013: PacBio (Sage Suite)- "Taking Advantage of Long Read Lengths with Improved Library Preparation Methods"
  • Ion World 2012: "Faster DNA size-selection on the Pippin Prep"
  • AGBT 2012: "The BluePippin System: Automated Size-selection of High Molecular Weight DNA"
  • AGBT 2012: Epicentre and Sage - "Improved RNA-seq Library Quality and Workflow Enabled by Automated Preparative Gel Electrophoresis"
  • AGBT 2012: Covaris and Sage - Complementary DNA Shearing and Size-selection Tools for Mate-pair Library Construction"
  • PAG 2012: Digilab and Sage - "Complementary DNA Shearing and Size-selection Tools for Mate-pair Library Construction"
  • AGBT 2011: "Expanded NGS Applications for the Pippin Prep DNA Size Selection System"
  • AGBT 2011: Workshop Presentation, Chris Boles - "Pippin Prep 2011: New Applications - Expanded Cassette Offerings and System Improvements"
  • AGBT 2010: "Automated preparative gel electrophoresis using the Pippin Prep system"
• Application Notes
  
• Case Studies
  
  • Case Study: "Tag Team: Scientists Use Pippin to Optimize Nextera Libraries", Zach Herbert, Dana Farber Core Facilities
  • Case Study: "Precise Sizing and SMRT Sequencing Offer Unprecedented Read Length for Clinical Studies", Robert Sebra, Mt. Sinai
  • Case Study: "Better Metagenomics Methods Offer Hope for Studies with Ultra-Low DNA", Sullivan Lab, Univ. Arizona
  • Case Study:" New Take on RADseq Enables High-Throughput Variant Discovery", Hoekstra Lab, Harvard Univ.

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