Pippin Prep

Short Read NGS Library Construction – For Inserts from 100bp to 1.5kb

The Pippin Prep facilitates library construction for the most popular NGS platforms, and is recommended by Illumina and Ion Torrent for certain workflows. The platform features the ability to collect narrow and even fragment distributions, as well as the flexibility to collect wide ranges of fragments with minimal effort. Target sizes or ranges of sizes are entered in software, and fractions are collected in buffer. Up to 5 samples per gel cassette may be run, with no possibility of cross contamination. Benefits of the Pippin Prep System:

• Saves time and effort compared to manual gel purification
• Sample-to-sample reproducibility streamlines research
• Higher sample quality improves downstream analysis

Key Downstream Technologies:

• Paired-end sequencing
• emPCR
• RNA-seq
• ChIP-seq

Pre-Cast Agarose Gel Cassettes for DNA Size Selection

Gel cassettes for DNA selections are available in four agarose concentrations to provide a full range of size selection options between 100bp and 1.5kb.  For smaller fragment sizes, internal standards may be used for higher accuracy and increased sample capacity (from 4 to 5 samples per cassette).  Run times are as little as 30 minutes. View the Pippin Prep cassette reference chart to explore the DNA size selection options and run times.

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Gel cassette types and target ranges

• 3% agarose, 100-250bp
• 2% agarose, 100-600bp
• 1.5% agarose, 250bp – 1.5kb

See the Workflow Summary.

For these downstream technologies

• miRNA isolation
• DNA sequencing, RNA-seq, genotyping
• DNA sequencing, ChIP-seq
• Mate-pair sequencing