Sage Science automated DNA sample prep tools are used in a wide range of applications. In each section below, we round up some of the great work our customers are doing.
Next-Gen Sequencing
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Publication: Spread of Chikungunya Virus East/Central/South African Genotype in Northeast Brazil, Emerging Infectious Diseases
Sage tool: Pippin Prep
Publication: Generation of Full-Length Class I Human Leukocyte Antigen Gene Consensus Sequences for Novel Allele Characterization, Clinical Chemistry
Sage tool: Pippin Prep
Publication: Improved Protocols for Illumina Sequencing, Current Protocols in Human Genetics
Sage tool: Pippin Prep
Publication: Enhanced Reduced Representation Bisulfite Sequencing for Assessment of DNA Methylation at Base Pair Resolution (video), JoVE
Sage tool: Pippin Prep
Customer blog: Creighton Lab Boosts Yield, Sequencing Efficiency with BluePippin, Creighton University
Sage tool: BluePippin
Customer blog: A Sage partnership, Broad Institute
Sage tool: Pippin Prep
Blog: TGAC Bioinformaticians Benefit from SageELF
Sage tools: BluePippin, SageELF
Blog: At Einstein Med School, ‘Extremely Reproducible’ Pippin Platforms Save Time and Money
Sage tools: Pippin Prep, PippinHT
Blog: 1,000th Pippin Customer: Cave Scientist Extraordinaire
Sage tool: Pippin Prep
Blog: For NRC Team, Pippin Platform Leads to Higher-Quality Assemblies
Sage tools: BluePippin, Pippin Prep
Case Study: Tag Team: Scientists Use Pippin to Optimize Nextera Libraries, Zach Herbert, Dana-Farber Cancer Institute
Sage tool: Pippin Prep
Case Study: Better Metagenomics Methods Offer Hope for Studies with Ultra-Low DNA, Matthew Sullivan, University of Arizona
Sage tool: Pippin Prep
Long-Read / Long-Range Sequencing
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Preprint: Mapping And Phasing Of Structural Variation In Patient Genomes Using Nanopore Sequencing, bioRxiv
Sage tool: PippinHT
Publication: Comprehensive comparison of Pacific Biosciences and Oxford Nanopore Technologies and their applications to transcriptome analysis, F1000Research
Sage tool: SageELF
Publication: PacBio-LITS: a large-insert targeted sequencing method for characterization of human disease-associated chromosomal structural variations, BMC Genomics
Sage tools: Pippin Prep, BluePippin
Publication: Assembly and diploid architecture of an individual human genome via single-molecule technologies, Nature Methods
Sage tool: BluePippin
Poster: Taking Advantage of Long Read Lengths with Improved Library Preparation Methods
Sage tool: BluePippin
Poster: Single Molecule, Real-Time Sequencing of Full-length cDNA Transcripts Uncovers Novel Alternatively Spliced Isoforms
Tool: SageELF
Customer blog: For 10x Genomics Workflow, Broad Institute Uses PippinHT Size Selection, Broad Institute
Sage tool: PippinHT
Customer blog: Longing for the longest reads: PacBio and BluePippin, Norwegian Sequencing Centre
Sage tool: BluePippin
Customer blog: Increasing PacBio RS II SubRead Lengths, Institute for Genome Sciences
Sage tool: BluePippin
Case Study: Precise Sizing and SMRT Sequencing Offer Unprecedented Read Length for Clinical Studies, Robert Sebra, Icahn School of Medicine at Mount Sinai
Sage tool: BluePippin
App note: The effect of size selection of PacBio long-fragment libraries (≧7 kb), National Cancer Center Institute, Japan
Sage tools: BluePippin, Pippin Pulse
App note: Long-fragment library preparation for PacBio RS II – P5-C3 chemistry, Okinawa Institute of Advanced Sciences
Sage tool: BluePippin
MicroRNA / RNA-seq
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Blog: PippinHT in the Wild: High-Throughput ChIP-Seq at Whitehead Core Lab
Sage tool: PippinHT
Blog: At MIT, Pippin Enables Splice Variant Detection and MicroRNA Analysis
Sage tool: Pippin Prep
Blog: At Iowa Core Lab, Pippin Sizing Is Essential for MicroRNA Studies
Sage tool: Pippin Prep
App note: Automated Size Selection of NEBNext Small RNA Libraries with the Sage Pippin Prep, New England Biolabs
Sage tool: Pippin Prep
Mate-Pair Sequencing
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Publication: A method to simultaneously construct up to 12 differently sized Illumina Nextera long mate pair libraries with reduced DNA input, time, and cost, Biotechniques
Sage tool: SageELF
Publication: An improved approach to mate-paired library preparation for Illumina sequencing
Sage tool: BluePippin
Publication: Optimization and cost-saving in tagmentation-based mate-pair library preparation and sequencing, Biotechniques
Sage tool: BluePippin
Blog: TGAC Bioinformaticians Benefit from SageELF
Sage tools: BluePippin, SageELF
Blog: For NRC Team, Pippin Platform Leads to Higher-Quality Assemblies
Sage tools: BluePippin, Pippin Prep
Blog: At TGAC, Pippin-Aided Sequencing Gives Quality Boost to Genome Assemblies
Sage tool: BluePippin
Blog: At Emory, Size Selection Saves Time for NGS Prep
Sage tool: Pippin Prep
App note: Size selection of long-chain fragments (8kb) in Nextera Mate Pair library preparation, University of Miyazaki, Japan
Sage tool: BluePippin
ChIP-seq
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Publication: Semiconductor-based DNA sequencing of histone modification states, Nature Communications
Sage tool: Pippin Prep
App note: ChIP-seq Library Prep, Dana-Farber Cancer Institute
Sage tool: Pippin Prep
Blog: PippinHT in the Wild: High-Throughput ChIP-Seq at Whitehead Core Lab
Sage tool: PippinHT
Blog: At Boston University, Pippin Makes Quick Work of ChIP-seq Prep
Sage tool: Pippin Prep
ddRADseq / Massively Parallel Genotyping
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Blog: At NYU’s Center for Genomics and Systems Biology, Pippin Allows for More Precise DNA Size Selection
Sage tool: Pippin Prep
Case Study: New Take on RADseq Enables High-Throughput Variant Discovery, Brant Peterson, Harvard University
Sage tool: Pippin Prep
