Bead Template Prep

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Improving Emulsion PCR libraries with Narrow Size Selections

In bead-based sequencing processes, emulsion PCR is performed to generate sequencing templates on the beads. As in other PCR processes, smaller DNA fragments will be more efficiently utilized in amplification and sequencing. If the library DNA is size selected on the Pippin Prep to produce a narrow size distribution before amplification, this bias can be reduced, and libraries with more uniform sequence representation can be produced.

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