Automated size selection can be an important step for getting the highest quality RNA-seq result. Sizing efficiently removes unwanted adapters and artifacts from libraries, while providing fragments sizes that best suited to the sequencing platform and reagent kit being used. Illumina users frequently use Pippin sizing for paired-end library construction for more efficient assemblies of transcripts and detection of variants. More…
The Iso-seq method for PacBio sequencing uses its long-read technology to identify splice variants in full-length RNA transcripts. This method uses either the SageELF or BluePippin to bin fragment sizes which significantly improves downstream analysis. More…
Check out our microRNA applications page for information about isolating small RNA libraries prior to Illumina sequencing.