Tag Archives: ILMN

quaddRAD: a new high-multiplexing and PCR duplicate removal ddRAD protocol produces novel evolutionary insights in a nonradiating cichlid lineage

May 2017 Authors: Franchini P, Monné Parera D, Kautt AF, Meyer A Info: Scientists from the University of Konstanz modified the original ddRAD-seq protocol to make it faster, more cost-effective, and higher-capacity. They report results from validating the new method … more »

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The RNA Exosome Syncs IAV-RNAPII Transcription to Promote Viral Ribogenesis and Infectivity

April 2017 Authors: Alexander Rialdi, Judd Hultquist, David Jimenez-Morales, Zuleyma Peralta, et al. Info: Scientists in New York and California teamed up to study the role of nuclear RNA exosomes in viral transcription, using influenza A. They found that the … more »

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Where Illumina Users Gather, Great Science Is on Display

The Sage team is pleased to be sponsoring the slate of upcoming Illumina user group meetings. We’ve attended many of these events over the years, and they’re excellent venues that showcase truly impressive work from the company’s broad customer base. … more »

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Optimization and cost-saving in tagmentation-based mate-pair library preparation and sequencing

May 2015 Authors: Kaori Tatsumi, Osamu Nishimura, Kazu Itomi, Chiharu Tanegashima, and Shigehiro Kuraku Info: Scientists from RIKEN modified the recommended protocol for the Nextera Mate Pair Sample Prep Kit from Illumina to improve library yield and quality while minimizing … more »

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BluePippin Optimized for Illumina’s Moleculo Kit

As Sage Science blog readers know, BluePippin is used throughout the genomics community for size-selecting larger fragments — we’ve gotten lots of attention for how well the tool performs with extremely long reads from the Pacific Biosciences sequencer, for example. … more »

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Illumina Workflow: Pippin for Improved Assembly Accuracy

As we’ve seen throughout this blog series, Sage customers are conducting all sorts of great experiments pairing their Pippin size selection instruments with Illumina sequencers. Today we look at the final topic in this thread: boosting assembly accuracy with precise … more »

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Illumina Workflow: Pippin for Massively Parallel Genotyping

With so many Sage customers using their Pippin instruments in an Illumina sequencer pipeline, we’re taking a look at various applications enabled by the Sage + Illumina combination. Today we check out double-digest RADseq, which could not work without precise … more »

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Illumina Workflow: Pippin for ChIP-seq

As we continue our blog series on applications that are frequently used with Pippin size selection and Illumina sequencing, we move on to ChIP-seq. One of the most popular capabilities enabled by next-gen sequencing, ChIP-seq (or chromatin immunoprecipitation sequencing) is … more »

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Illumina Workflow: Pippin with Nextera

In this blog series, we’ve been looking at how Sage Science customers use their Pippin Prep and BluePippin instruments with their Illumina sequencers. Today we check out the Nextera workflow, which is designed for speedy NGS sample prep. It can … more »

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Illumina Workflow: Pippin for RNA Studies

Illumina sequencers are far and away the most popular platform on the market. Since so many Sage customers use their Pippins with these instruments, we’re taking a look at some of the most common or interesting applications of automated DNA … more »

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