Sage at Consumer Genetics Conference, Boston

Chris Boles, Chief Scientific Officer at Sage Science will be speaking about the Pippin Prep at the Consumer Genetic Conference at the Hynes Covention Center in Boston, MA on June 2, 2010.

Sage at AGBT, Marco Island

Sage Science attends the Advances in Genome Biology and Technology (AGBT) conference in Marco Island FL,  Feb. 25-27 2010.

download AGBT poster

paired-end sequencing

NGS library construction

restriction fragment isolation

PCR band collection

sub-cloning

Each disposable cassette has 4 sample lanes and 1 lane for a DNA reference ladder (provided by Sage).  The sample lanes are physically separate, preventing any possibility of cross-contamination.  Size ranges to be extracted are entered in software, and a non-UV optical detection system tracks the DNA migration and correctly times the electro-elution “cut”.

run times:               50 – 100 min

loading vol:              40 ul

elution vol:               40-60 ul

loading  capacity:     up to20 ug of sheared genomic DNA

                               up to 200 ng of PCR product

min. cut range:         <4% CV (randomly sheared genomic DNA)

recovery efficiency:   >50%